A5: Molecular switches driving Th17 cells into IL-17+Treg cells as mediators of immune tolerance to allergens
The switch between Th17 and Tregs is also the switch between health and disease and therefore a very major topic not only in allergy and autoimmune disease. More recently we also observe that Tregs and Tr17 cells occur on site of local inflammation in the airway lumen and therefore intend to analyze the function of the cells in fascinating organoid cultures. These models are suitable to explore the function of novel genes that are coming from our single cells sequencing studies (hot and unknown), but they also represent novel targets for diagnosis and treatment of diseases. Technologies are: Cell culture, organoid cultures, quantitative PCR, single cell seq, DNA array technology (mRNA, miRNA, methlome), high dimensional flow cytometry, western blot, targeted metabolomics, mesoscale protein analysis, Luminex, fluorescent immunohistology, life cell imaging, optional also murine disease models including sophisticated tracer systems). For both tandem team member this project provides an institute and supervision 100% dedicated for research.